Combination of chromatographic techniques for the preparative isolation of anthocyanins -: applied on blackcurrant (Ribes nigrum) fruits

A combination of column chromatography on Toyopearl HW-40F gel and reversed-phase high-performance Liquid chromatography enabled us to preparatively separate anthocyanins, without any re-application of overlapping bands of the major (the 3-O-glucosides and 3-O-rutinosides of delphinidin and cyanidin) and minor (the 3-O-rutinosides of peonidin and malvidin) anthocyanins in a 0.81-g sample from blackcurrant (Ribes nigrum) fruits. Anthocyanins substituted with methoxyl groups on the aglycone have never been detected previously in the genus Ribes. By variation of sample loading, flow-rate and solvent strength, Toyopearl HW-40F gel gave rise to chromatograms with higher resolution between the anthocyanidin 3-rutinoside and anthocyanidin 3-glucoside bands than Sephadex LH-20 gel, in all cases. (C) 1998 Elsevier Science BN. All rights reserved.