Grouping newly isolated docosahexaenoic acid-producing thraustochytrids based on their polyunsaturated fatty acid profiles and comparative analysis of 18S rRNA genes

被引：76

作者：

Huang, JZ

Aki, T ^{[1
]}

Yokochi, T

Nakahara, T

Honda, D

Kawamoto, S

Shigeta, S

Ono, K

Suzuki, O

机构：

[1] Hiroshima Univ, Dept Mol Biotechnol, Grad Sch Adv Sci Matter, Higashihiroshima 7398530, Japan

[2] Natl Inst AIST, Biol Resources Lab, Ibaraki 3058566, Japan

[3] Konan Univ, Fac Sci & Engn, Dept Biol, Kobe, Hyogo 6588501, Japan

来源：

MARINE BIOTECHNOLOGY | 2003年 / 5卷 / 05期

关键词：

18S rRNA gene; docosahexaenoic acid; molecular phylogeny; polyunsaturated fatty acids; Schizochytrium; Thraustochytrium;

D O I：

10.1007/s10126-002-0110-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Seven strains of marine microbes producing a significant amount of docosahexaenoic acid (DHA; C22:6, n-3) were screened from seawater collected in coastal areas of Japan and Fiji. They accumulate their respective intermediate fatty acids in addition to DHA. There are 5 kinds of polyunsaturated fatty acid (PUFA) profiles which can be described as (1) DHA/docosapentaenoic acid (DPA; C22:5, n-6), (2) DHA/DPA/eicosapentaenoic acid (EPA; C20:5, n-3), (3) DHA/EPA, (4) DHA/DPA/EPA/arachidonic acid (AA; C20:4, n-6), and (5) DHA/DPA/EPA/AA/docosatetraenoic acid (C22:4, n-6). These isolates are proved to be new thraustochytrids by their specific insertion sequences in the 18S rRNA genes. The phylogenetic tree constructed by molecular analysis of 18S rRNA genes from the isolates and typical thraustochytrids shows that strains with the same PUFA profile form each monophyletic cluster. These results suggest that the C20-22 PUFA profile may be applicable as an effective characteristic for grouping thraustochytrids.

引用

页码：450 / 457

页数：8

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