Optimized serodiagnosis of Mycoplasma pneumoniae infections

被引：25

作者：

Dumke, Roger ^{[1
]}

Strubel, Andreas ^{[2
]}

Cyncynatus, Camille ^{[3
]}

Nuyttens, Helene ^{[3
]}

Herrmann, Richard ^{[4
]}

Lueck, Christian ^{[1
]}

Jacobs, Enno ^{[1
]}

机构：

[1] Tech Univ Dresden, Inst Med Microbiol & Hyg, D-01307 Dresden, Germany

[2] Sekisui Virotech GmbH, D-65428 Russelsheim, Germany

[3] Gen BioSci, F-91380 Chilly Mazarin, France

[4] Heidelberg Univ, ZMBH, D-69120 Heidelberg, Germany

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2012年 / 73卷 / 02期

关键词：

Mycoplasma pneumoniae; Immunoblot; Community-acquired pneumonia; Recombinant proteins; COMMERCIAL TESTS; P1; ADHESIN; PROTEIN; IDENTIFICATION; ANTIBODIES; DIAGNOSIS; EXPRESSION; P200; PCR;

D O I：

10.1016/j.diagmicrobio.2012.02.014

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Serologic methods are well established for the diagnosis of Mycoplasma pneumoniae infections in humans, but they are less sensitive than polymerase chain reaction (PCR). To improve their sensitivity, a new panel of antigens was tested. Compared with PCR results, up to 92% of PCR-positive patients were confirmed by our immunoblotting approach having a specificity between 92.6% and 100%. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：200 / 203

页数：4

共 23 条

[1] Evaluation of 12 commercial tests and the complement fixation test for Mycoplasma pneumoniae-specific immunoglobulin G (IgG) and IgM antibodies, with PCR used as the "gold standard" [J].

Beersma, MFC ;

Dirven, K ;

van Dam, AP ;

Templeton, KE ;

Claas, ECJ ;

Goossens, H .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (05) :2277-2285

[2] Expression and immunological characterization of the carboxy-terminal region of the P1 adhesin protein of Mycoplasma pneumoniae [J].

Chaudhry, R ;

Nisar, N ;

Hora, B ;

Chirasani, SR ;

Malhotra, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (01) :321-325

[3] Comparison of four Mycoplasma pneumoniae IgM-, IgG- and IgA-specific enzyme immunoassays in blood donors and patients [J].

Csángó, PA ;

Pedersen, JE ;

Hess, RD .

CLINICAL MICROBIOLOGY AND INFECTION, 2004, 10 (12) :1094-1098

[4] Re-annotating the Mycoplasma pneumoniae genome sequence:: adding value, function and reading frames [J].

Dandekar, T ;

Huynen, M ;

Regula, JT ;

Ueberle, B ;

Zimmermann, CU ;

Andrade, MA ;

Doerks, T ;

Sánchez-Pulido, L ;

Snel, B ;

Suyama, M ;

Yuan, YP ;

Herrmann, R ;

Bork, P .

NUCLEIC ACIDS RESEARCH, 2000, 28 (17) :3278-3288

[5] Laboratory diagnosis of Mycoplasma pneumoniae infection [J].

Daxboeck, F ;

Krause, R ;

Wenisch, C .

CLINICAL MICROBIOLOGY AND INFECTION, 2003, 9 (04) :263-273

[6] Immune response to Mycoplasma pneumoniae P1 and P116 in patients with atypical pneumonia analyzed by ELISA -: art. no. 7 [J].

Drasbek, M ;

Nielsen, PK ;

Persson, K ;

Birkelund, S ;

Christiansen, G .

BMC MICROBIOLOGY, 2004, 4 (1) :1-10

[7] Characterisation of subtype- and variant-specific antigen regions of the P1 adhesin of Mycoplasma pneumoniae [J].

Dumke, Roger ;

Schurwanz, Nicol ;

Jacobs, Enno .

INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2008, 298 (5-6) :483-491

[8] Comparison of Commercial and In-House Real-Time PCR Assays Used for Detection of Mycoplasma pneumoniae [J].

Dumke, Roger ;

Jacobs, Enno .

JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (02) :441-444

[9] Mycoplasma pneumoniae infections [J].

Hammerschlag, MR .

CURRENT OPINION IN INFECTIOUS DISEASES, 2001, 14 (02) :181-186

[10] Protein P200 is dispensable for Mycoplasma pneumoniae hemadsorption but not gliding motility or colonization of differentiated bronchial epithelium [J].

Jordan, Jarrat L. ;

Chang, How-Yi ;

Balish, Mitchell F. ;

Holt, Lynley S. ;

Bose, Stephanie R. ;

Hasselbring, Benjamin M. ;

Waldo, Robert H., III ;

Krunkosky, Thomas M. ;

Krause, Duncan C. .

INFECTION AND IMMUNITY, 2007, 75 (01) :518-522

← 1 2 3 →