Boric acid as a mobile phase additive for high performance liquid chromatography separation of ribose, arabinose and ribulose

被引:37
作者
De Muynck, C [1 ]
Beauprez, J [1 ]
Soetaert, W [1 ]
Vandamme, EJ [1 ]
机构
[1] Univ Ghent, Dept Biochem & Microbial Technol, Lab Ind Microbiol & Biocatalysis, B-9000 Ghent, Belgium
关键词
carbohydrate analysis; boric acid; ribose; ribulose; arabinose; HPLC;
D O I
10.1016/j.chroma.2005.09.068
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A new high performance liquid chromatographic (HPLC) method is described for the analysis of ribose, arabinose and ribulose mixtures obtained from (bio)chemical isomerization processes. These processes gain importance since the molecules can be used for the synthesis of antiviral therapeutics. The HPLC method uses boric acid as a mobile phase additive to enhance the separation on an Aminex HPX-87K column. By complexing with boric acid, the carbohydrates become negatively charged, thus elute faster from the column by means of ion exlusion and are separated because the complexation capacity with boric acid differs from one carbohydrate to another. Excellent separation between ribose, ribulose and arabinose was achieved with concentrations between 0.1 and 10 g L-1 of discrete sugar. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:115 / 121
页数:7
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