Lateral resolution of 28 nm (λ/25) in far-field fluorescence microscopy

被引：69

作者：

Westphal, V ^{[1
]}

Kastrup, L ^{[1
]}

Hell, SW ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept NanoBiophoton, D-37077 Gottingen, Germany

来源：

APPLIED PHYSICS B-LASERS AND OPTICS | 2003年 / 77卷 / 04期

关键词：

D O I：

10.1007/s00340-003-1280-x

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We demonstrate sub-diffraction lateral resolution of 28 +/- 2 nm in far-field fluorescence microscopy through stimulated emission depletion effected by an amplified laser diode. Measurement of the optical transfer function in the focal plane reveals a 6-fold enlargement of the spatial bandwith over the diffraction limit. The resolution is established by imaging individual fluorescent molecules on a surface. Corresponding to 1/25 of the responsible wavelength, the attained resolution represents a new benchmark in far-field microscopy and underscores the viability of fluorescence nanoscopy with visible light, conventional optics and compact laser systems.

引用

页码：377 / 380

页数：4

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