Poly(ethylene glycol)-block-poly(L-lysine) dendrimer:: Novel linear polymer/dendrimer block copolymer forming a spherical water-soluble polyionic complex with DNA

被引：133

作者：

Choi, JS ^{[1
]}

Lee, EJ ^{[1
]}

Choi, YH ^{[1
]}

Jeong, YJ ^{[1
]}

Park, JS ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Chem, Kwanak Ku, Seoul 151742, South Korea

来源：

BIOCONJUGATE CHEMISTRY | 1999年 / 10卷 / 01期

关键词：

D O I：

10.1021/bc9800668

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Methoxypoly(ethylene glycol)-block-poly(L-lysine) dendrimer was designed to form a water-soluble complex with plasmid DNA. The copolymer was synthesized by the liquid-phase peptide synthesis method. It was characterized by H-1 NMR and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrum. Agarose gel electrophoresis and DNase I protection assay proved that this linear polymer/dendrimer block copolymer assembled spontaneously with plasmid DNA, forming a water-soluble complex which increased the stability of the complexed DNA. Atomic force microscopy of the complex was evaluated at various charge ratios showing that the copolymer/DNA complex was like a globular shape.

引用

页码：62 / 65

页数：4