Liquid chromatography of troleandomycin

被引：16

作者：

Chepkwony, HK ^{[1
]}

Roets, E ^{[1
]}

Hoogmartens, J ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Fac Farmaceut Wetenschappen, Lab Farmaceut Chem Analyse Van Geneesmiddelen, B-3000 Louvain, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 914卷 / 1-2期

关键词：

troleandomycin; antibiotics;

D O I：

10.1016/S0021-9673(00)01170-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Until now no liquid chromatography (LC) method is described to determine the purity and content of troleandomycin and its related substances. A simple, robust, sensitive and selective isocratic liquid chromatographic method suitable for the determination of the antibiotic troleandomycin and its related substances is described. This method utilizes as a stationary phase: XTerra RP18 5 mum (25 cmX4.6 mm I.D.) at 30 degreesC and as mobile phase: acetonitrile-0.2 M ammonium acetate buffer (pH 6.0)-water (45:5:50, v/v), delivered at a flow-rate of 1.0 ml/min. W detection is performed at 205 nm. Troleandomycin is separated from the partially acetylated related substances and from several unknown impurities present in commercial samples. The robustness of the method was evaluated by a full-factorial experimental design. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：53 / 58

页数：6

共 9 条

[1]

BRYSKIER A, 1993, MACROLIDES CHEM PHAR, P12

[2] DETERMINATION OF ERYTHROMYCIN ETHYLSUCCINATE BY LIQUID-CHROMATOGRAPHY [J].