Liquid chromatography of josamycin propionate on poly(styrene-divinylbenzene)

被引：13

作者：

Roets, E ^{[1
]}

Lepoudre, X ^{[1
]}

Van Rompaey, V ^{[1
]}

Velghe, G ^{[1
]}

Liu, L ^{[1
]}

Hoogmartens, J ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Fac Farmaceut Wetenschappen, Lab Farmaceut Chem Anal Geneesmiddelen, B-3000 Leuven, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 812卷 / 1-2期

关键词：

pharmaceutical analysis; josamycin propionate; antibiotics;

D O I：

10.1016/S0021-9673(98)00123-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An isocratic liquid chromatographic method is described using as stationary phase the very stable poly(styrene-divinylbenzene) PLRP-S (8 mu m, 1000 Angstrom). The mobile phase was acetonitrile-0.2 M phosphate buffer, pH 10.0-water (52:20:up to 100, v/v/v), delivered at a flow-rate of 1.0 ml/min. UV detection was performed at 232 nm. The column was heated at 60 degrees C. Josamycin propionate was separated from all impurities which were present in commercial samples. The main impurities were josamycin, leucomycin A4 propionate, josamycin 2',9-dipropionate, josamycin 3 ",9-dipropionate and platenomycin Al propionate. Full factorial design was applied to evaluate the robustness of the method. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：303 / 308

页数：6

共 8 条

[1] Liquid chromatography of spiramycin on poly(styrene-divinylbenzene) [J].