LIQUID-CHROMATOGRAPHY OF ERYTHROMYCIN-A AND RELATED SUBSTANCES ON POLY(STYRENE-DIVINYLBENZENE)

被引：49

作者：

PAESEN, J ^{[1
]}

ROETS, E ^{[1
]}

HOOGMARTENS, J ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN,INST FARMACEUT WETENSCHAPPEN,FARMACEUT CHEM LAB,VAN EVENSTR 4,B-3000 LOUVAIN,BELGIUM

来源：

CHROMATOGRAPHIA | 1991年 / 32卷 / 3-4期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; ERYTHROMYCIN-A AND RELATED SUBSTANCES; POLY(STYRENE-DIVINYLBENZENE);

D O I：

10.1007/BF02325020

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An isocratic liquid chromatography method for assay and purity control of erythromycin is presented. Erythromycin A is separated from all its potential impurities, except erythromycin D. The selectivity depends on the pore size of the poly(styrene-divinylbenzene) stationary phase. Wide pore PLRP-S 8-mu-m 1000 angstrom shows the best selectivity. The column is heated at 70-degrees-C. The mobile phase is acetonitrile -2-methyl-2-propanol-0.2 M potassium phosphate buffer pH 9.0-water (3:16.5:5:75.5). The flow rate is 2 ml/min. UV detection is performed at 215 nm. The total analysis time is about 30 min. The method was used to compare official standards.

引用

页码：162 / 166

页数：5

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