LIQUID-CHROMATOGRAPHY OF JOSAMYCIN ON POLY(STYRENE-DIVINYLBENZENE)

被引：11

作者：

PAESEN, J ^{[1
]}

SOLIE, A ^{[1
]}

ROETS, E ^{[1
]}

HOOGMARTENS, J ^{[1
]}

机构：

[1] KATHOLIEKE UNIV LEUVEN,FAC FARMACEUT WETENSCHAPPEN,FARMACEUT CHEM ANALYSE GENEESMIDDELEN LAB,B-3000 LOUVAIN,BELGIUM

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1995年 / 352卷 / 7-8期

关键词：

D O I：

10.1007/BF00323067

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An isocratic liquid chromatographic method for the assay and purity control of josamycin using wide-pore poly(styrene-divinylbenzene) as the stationary phase is presented. Josamycin has been well separated from all the related leucomycins, present as impurities in commercial samples. Wide-pore PLRP-S 8 mu m 1000 A has shown the best selectivity. The separation has been carried out at 65 degrees C with a mobile phase of 2-methyl-2-propanol/0.2 mol/l potassium phosphate buffer pH 10.5/water (24.5: 5: 70.5, V/V) and a flow rate of 1.0 ml/min. UV detection has been performed at 232 nm. The total analysis time has been about 40 min. The method has been used to analyse commercial bulk samples and tablets containing josamycin base.

引用

页码：797 / 800

页数：4

共 9 条

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OMURA S, 1968, CHEM PHARM BULL, V16, P1181

[2] FAST LIQUID CHROMATOGRAPHY OF MACROLIDE ANTIBIOTICS [J].