LIQUID-CHROMATOGRAPHY OF TYLOSIN-A AND RELATED SUBSTANCES ON POLY(STYRENE-DIVINYLBENZENE)

被引：18

作者：

PAESEN, J ^{[1
]}

CLAEYS, P ^{[1
]}

CYPERS, W ^{[1
]}

ROETS, E ^{[1
]}

HOOGMARTENS, J ^{[1
]}

机构：

[1] KATHOLIEKE UNIV LEUVEN,FAC FARMACEUT WETENSCHAPPEN,FARMACEUT CHEM & ANAL GENEESMIDDELEN LAB,B-3000 LOUVAIN,BELGIUM

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 699卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)01268-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A liquid chromatographic method for analysis of tylosin A and related substances was developed using wide-pore poly(styrene-divinylbenzene) as the stationary phase. As a mobile phase, a mixture of tetrahydrofuran-0.2 M potassium phosphate buffer pH 9.0-water (20:5:75) was used at a flow-rate of 1.0 ml/min. The column was kept at 60 degrees C. Detection was performed by UV at 280 nm. Tylosin A is separated from all its known related substances that are potentially present as impurities in commercial samples of tylosin base and tylosin tartrate. The method shows good linearity and repeatability and is stability indicating. Results for a number of commercial samples analysed using the newly developed method are presented.

引用

页码：93 / 97

页数：5

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