Regulation of apoptotic pathways in bovine γ/δ T cells

被引：5

作者：

Deng, MQ ^{[1
]}

Liu, J ^{[1
]}

Pelak, CN ^{[1
]}

Lancto, CA ^{[1
]}

Abrahamsen, MS ^{[1
]}

机构：

[1] Univ Minnesota, Coll Vet Med, Dept Vet & Biomed Sci, St Paul, MN 55108 USA

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2005年 / 105卷 / 1-2期

关键词：

gamma/delta T cell; apoptosis; microarray; real-time PCR; mitogenic stimulation;

D O I：

10.1016/j.vetimm.2004.11.011

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

T lymphocytes bearing gamma/delta TCRs are a major population of T cells in neonatal calves and discrete subsets of gamma/delta T cells display tissue-specific accumulation and responsiveness to infection. To enhance our understanding of the immunobiology of gamma/delta 8 T cells, we characterized the gene expression profile of circulating bovine gamma/delta T cells following stimulation with recombinant human IL-2 and ConA. Statistical analysis of microarray data identified 108 genes with significantly altered expression, including four genes associated with apoptosis. Real-time reverse transcription-PCR (RT-PCR) analysis of 15 genes related to apoptotic pathways showed that both the Fas-mediated and the mitochondrial apoptotic pathways were repressed in circulating bovine gamma/delta T cells in response to mitogen activation, indicating that stimulated peripheral bovine gamma/delta T cells are resistant to activation-induced apoptosis. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：15 / 23

页数：9

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