Polarity of microtubule assemblies during neuronal cell migration

被引:103
作者
Rakic, P
KnyiharCsillik, E
Csillik, B
机构
[1] Section of Neurobiology, Yale University, School of Medicine, New Haven
[2] Department of Neurology, Albert Szent-Gyorgyi Med. University, Szeged
[3] Bay Zoltan Inst. for Biotechnology, Szeged
关键词
neural development; cerebellar granule cells; cell motility;
D O I
10.1073/pnas.93.17.9218
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The active migration of neurons from their sites of origin to their final destinations requires the unidirectional translocation of the nuclei and somatic cytoplasm within the growing leading processes. To explore the cellular machinery underlying this translocation, we determined the polarity of microtubules situated within the leading and trailing processes of migrating cerebellar granule cells in situ. Our analysis reveals that the newly assembled positive ends of the microtubules in the leading process uniformly face the growing tip, while their disintegrating negative ends face the nucleus. In the trailing process, by contrast, microtubule arrays are of mixed polarity. We suggest that the dynamics of slow polymerization in combination with fast disintegration of oriented microtubules create ''push'' and ''pull'' forces that contribute to the piston-like saltatory displacement of the nucleus and cytoplasm within the membrane cylinder of the leading process of the migrating neuron.
引用
收藏
页码:9218 / 9222
页数:5
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