Minimal tRNASer and tRNASec substrates for human seryl-tRNA synthetase:: contribution of tRNA domains to serylation and tertiary structure

被引：15

作者：

Heckl, M ^{[1
]}

Busch, K ^{[1
]}

Gross, HJ ^{[1
]}

机构：

[1] Univ Wurzburg, Inst Biochem, D-97074 Wurzburg, Germany

来源：

FEBS LETTERS | 1998年 / 427卷 / 03期

关键词：

tRNA(Ser); tRNA(Sec); seryl-tRNA synthetase; microhelix; RNA structure; RNA structure probing;

D O I：

10.1016/S0014-5793(98)00435-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The recognition process of tRNA(Ser) and tRNA(Sec) by human seryl-tRNA synthetase (SerRS) was studied using T7 transcripts representing defined regions of human tRNA(Ser) or tRNA(Sec) and the influence of the tRNA elements on serylation and tertiary structure was elucidated, The anticodon arms of both tRNAs showed no contribution to serylation in contrast to the acceptor stems and the long extra arms, D and T arms mere only involved in formation of the L-shaped tRNA structure, not in the recognition process between tRNAs and SerRS, This is the first report of microhelices adapted from human tRNAs being aminoacylated by their homologous synthetase, (C) 1998 Federation of European Biochemical Societies.

引用

页码：315 / 319

页数：5

共 35 条

[1] IDENTITY DETERMINANTS OF HUMAN TRANSFER RNA(SER) - SEQUENCE ELEMENTS NECESSARY FOR SERYLATION AND MATURATION OF A TRANSFER-RNA WITH A LONG EXTRA ARM [J].