Detection of target DNA by electrochemical method

被引：49

作者：

Maruyama, K

Motonaka, J

Mishima, Y

Matsuzaki, Y

Nakabayashi, I

Nakabayashi, Y

机构：

[1] Univ Tokushima, Dept Chem Sci & Technol, Fac Engn, Tokushima 7708506, Japan

[2] Univ Tokushima, Fac Pharmaceut Sci, Tokushima 7708505, Japan

[3] Kansai Univ, Fac Engn, Suita, Osaka 5648680, Japan

[4] Kansai Univ, HRC, Suita, Osaka 5648680, Japan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2001年 / 76卷 / 1-3期

关键词：

DNA; osmium complex; electrochemical detection; square wave voltammetry;

D O I：

10.1016/S0925-4005(01)00645-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The interaction of the osmium complexes {[Os(bpy)(3)](3+) (bpy = bipyridine), [Os(phen)(3)](2+) (phen = 1,10-phenanthroline), [Os(5,6-dmphen)(3)](2+) (5,6-dmphen = 5,6-dimethyl-1, 10-phenanthroline), and [Os(4,7-dmphen)(3)](2+) (4,7-dmphen = 4,7-dimethyl- 1,10-phenanthroline)} with calf thymus DNA were studied electrochemically. The binding constant of [Os(5,6-dmphen)(3)](2+), K = 4.2 x 10(5) M-1 was found to be larger than those observed for the known metal complex indicator and osmium complexes with methyl phenanthroline ligands. This paper shows that a DNA sensor system with [Os(5,6-dmphen)(3)](2+) as a hybridization indicator and square wave voltammetry, could selectively detect DNA in a range of 6.9 x 10(-10) to 6.9 x 10(-5) g/ml. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：215 / 219

页数：5

共 13 条

[11] DNA electrochemical biosensor for the detection of short DNA sequences related to the human immunodeficiency virus [J].