Murine branched chain α-ketoacid dehydrogenase kinase;: cDNA cloning, tissue distribution, and temporal expression during embryonic development

These studies were designed to demonstrate the structural and functional similarity of murine branched chain a-ketoacid dehydrogenase and its regulation by the complex-specific kinase. Nucleotide sequence and deduced amino acid sequence for the kinase cDNA demonstrate a highly conserved coding sequence between mouse and human. Tissue-specific expression in adult mice parallels that reported in other mammals. Kinase expression in female liver is influenced by circadian rhythm. Of special interest is the fluctuating expression of this kinase during embryonic development against the continuing increase in the catalytic subunits of this mitochondrial complex during development. The need for regulation of the branched chain a-ketoacid dehydrogenase complex by kinase expression during embryogenesis is not understood. However, the similarity of murine branched chain a-ketoacid dehydrogenase and its kinase to the human enzyme supports the use of this animal as a model for the human system. (C) 1998 Elsevier Science B.V. All rights reserved.