Histone H2A/H2B dimer exchange by ATP-dependent chromatin remodeling activities

被引:159
作者
Bruno, M [1 ]
Flaus, A [1 ]
Stockdale, C [1 ]
Rencurel, C [1 ]
Ferreira, H [1 ]
Owen-Hughes, T [1 ]
机构
[1] Univ Dundee, Sch Life Sci, Div Gene Regulat & Express, Dundee DD1 5EH, Scotland
基金
英国惠康基金;
关键词
D O I
10.1016/S1097-2765(03)00499-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
ATP-dependent chromatin remodeling activities function to manipulate chromatin structure during gene regulation. One of the ways in which they do this is by altering the positions of nucleosomes along DNA. Here we provide support for the ability of these complexes to move nucleosomes into positions in which DNA is unraveled from one edge. This is expected to result in the loss of histone-DNA contacts that are important for retention of one H2A/H2B dimer within the nucleosome. Consistent with this we find that several chromatin remodeling complexes are capable of catalyzing the exchange of H2A/H2B dimers between chromatin fragments in an ATP-dependent reaction. This provides eukaryotes with additional means by which they may manipulate chromatin structure.
引用
收藏
页码:1599 / 1606
页数:8
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