Phototropins But Not Cryptochromes Mediate the Blue Light-Specific Promotion of Stomatal Conductance, While Both Enhance Photosynthesis and Transpiration under Full Sunlight

被引:76
作者
Boccalandro, Hernan E. [1 ,2 ,3 ]
Giordano, Carla V. [4 ]
Ploschuk, Edmundo L. [5 ]
Piccoli, Patricia N. [1 ,2 ]
Bottini, Ruben [1 ,2 ]
Casal, Jorge J. [6 ]
机构
[1] Univ Nacl Cuyo, Inst Biol Agr Mendoza, Fac Ciencias Agr, RA-5507 Chacras De Coria, Argentina
[2] Consejo Nacl Invest Cient & Tecn CONICET, RA-5507 Chacras De Coria, Argentina
[3] Univ Nacl Cuyo, Inst Ciencias Basicas, RA-5500 Mendoza, Argentina
[4] Consejo Nacl Invest Cient & Tecn, Inst Argentino Invest Zonas Aridas, Ctr Cient Tecnol, RA-5500 Mendoza, Argentina
[5] Univ Buenos Aires, Catedra Cultivos Ind, Fac Agron, RA-1417 Buenos Aires, DF, Argentina
[6] Univ Buenos Aires, Inst Invest Fisiol & Ecol Vinculadas Agr, Fac Agron, RA-1417 Buenos Aires, DF, Argentina
关键词
WATER-USE EFFICIENCY; ARABIDOPSIS-THALIANA; ABSCISIC-ACID; PHYTOCHROME B; RESPONSES; MUTANTS; PROTEIN; LEAVES; LOCUS; PHOT1;
D O I
10.1104/pp.111.187237
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Leaf epidermal peels of Arabidopsis (Arabidopsis thaliana) mutants lacking either phototropins 1 and 2 (phot1 and phot2) or cryptochromes 1 and 2 (cry1 and cry2) exposed to a background of red light show severely impaired stomatal opening responses to blue light. Since phot and cry are UV-A/blue light photoreceptors, they may be involved in the perception of the blue light-specific signal that induces the aperture of the stomatal pores. In leaf epidermal peels, the blue light-specific effect saturates at low irradiances; therefore, it is considered to operate mainly under the low irradiance of dawn, dusk, or deep canopies. Conversely, we show that both phot1 phot2 and cry1 cry2 have reduced stomatal conductance, transpiration, and photosynthesis, particularly under the high irradiance of full sunlight at midday. These mutants show compromised responses of stomatal conductance to irradiance. However, the effects of phot and cry on photosynthesis were largely nonstomatic. While the stomatal conductance phenotype of phot1 phot2 was blue light specific, cry1 cry2 showed reduced stomatal conductance not only in response to blue light, but also in response to red light. The levels of abscisic acid were elevated in cry1 cry2. We conclude that considering their effects at high irradiances cry and phot are critical for the control of transpiration and photosynthesis rates in the field. The effects of cry on stomatal conductance are largely indirect and involve the control of abscisic acid levels.
引用
收藏
页码:1475 / 1484
页数:10
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