A novel role for proliferin-2 in the ex vivo expansion of hematopoietic stem cells

被引：19

作者：

Choong, ML

Tan, MCL

Luo, B

Lodish, HF

机构：

[1] Ctr Bioproc Technol, Singapore 117597, Singapore

[2] MIT, Whitehead Inst Biomed Res, Cambridge, MA 02139 USA

来源：

FEBS LETTERS | 2003年 / 550卷 / 1-3期

关键词：

hematopoietic progenitor cell; stromal cell; bone marrow; fetal liver; in vitro expansion;

D O I：

10.1016/S0014-5793(03)00844-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A family of proliferin genes was discovered on a microarray analysis of hematopoiesis supportive stromal cell lines. Proliferin-2 (PLF2) increased the frequency of long-term culture-initiating cells (LTC-IC) from 1 in 340 to 1 in 256 of the primary hematopoietic stem cell (HSC)-enriched bone marrow cells grown on MS5.1 feeder layer. A repeat using AFT024 feeder layer also showed a similar increase in LTG-IC (from 1 in 386 cells to 1 in 260 cells). The clonogenic output of the LTC-ICs was also increased significantly. The growth of various hematopoietic and stromal cell lines treated with PLF2 was found to increase by 4-27%, as measured by cell count and DNA synthesis assay. These findings open up the possibility of using PLF2 as a new member of the growth factor cocktails for the ex vivo expansion of HSC. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

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页码：155 / 162

页数：8

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