Cytokinin regulates type-A Arabidopsis response regulator activity and protein stability via two-component phosphorelay

被引:211
作者
To, Jennifer P. C. [1 ]
Deruere, Jean [1 ]
Maxwell, Bridey B. [1 ]
Morris, Veronica F. [1 ]
Hutchison, Claire E. [1 ]
Ferreira, Fernando J. [1 ]
Schaller, G. Eric [2 ]
Kieber, Joseph J. [1 ]
机构
[1] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA
[2] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA
关键词
D O I
10.1105/tpc.107.052662
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The plant hormone cytokinin regulates many aspects of growth and development. Cytokinin signaling involves His kinase receptors that perceive cytokinin and transmit the signal via a multistep phosphorelay similar to bacterial two-component signaling systems. The final targets of this phosphorelay are a set of Arabidopsis thaliana Response Regulator (ARR) proteins containing a receiver domain with a conserved Asp phosphorylation site. One class of these, the type-A ARRs, are negative regulators of cytokinin signaling that are rapidly transcriptionally upregulated in response to cytokinin. In this study, we tested the role of phosphorylation in type-A ARR function. Our results indicate that phosphorylation of the receiver domain is required for type-A ARR function and suggest that negative regulation of cytokinin signaling by the type-A ARRs most likely involves phosphorylation-dependent interactions. Furthermore, we show that a subset of the type-A ARR proteins are stabilized in response to cytokinin in part via phosphorylation. These studies shed light on the mechanism by which type-A ARRs act to negatively regulate cytokinin signaling and reveal a novel mechanism by which cytokinin controls type-A ARR function.
引用
收藏
页码:3901 / 3914
页数:14
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