A novel ferritin gene, SferH-5, reveals heterogeneity of the 26.5-kDa subunit of soybean (Glycine max) seed ferritin

被引：10

作者：

Dong, Xiangbai

Sun, Qiming

Wei, Dongping

Li, Jiahuang

Li, Jie

Tang, Bo

Jia, Qi

Hu, Wenjun

Zhao, Yongjuan

Hua, Zi-Chun ^{[1
]}

机构：

[1] Nanjing Univ, Coll Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China

来源：

FEBS LETTERS | 2007年 / 581卷 / 30期

基金：

中国国家自然科学基金;

关键词：

soybean seed ferritin; the 26.5-kDa subunit; gene cloning; functional characterization; heterogeneity;

D O I：

10.1016/j.febslet.2007.11.049

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel ferritin cDNA, SferH-5, has been cloned from 7-day-old soybean seedlings. Putative SferH-5 has 96% identity with SferH-1 reported previously. All the five amino acid variants distributed in the mature region are not involved in highly conserved residues associated with ferroxidase activity center. We speculate that SferH-5 encodes a novel 26.5-kDa subunit of soybean seed ferritin, which is designated H-5 in this study. Recombinant H-5 was able to assemble, together with co-expressed H-2, as a functional soybean seed ferritin-like complex, H-5/H-2. Our data reveal the potential heterogeneity of the 26.5-kDa subunit of soybean seed ferritin. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：5796 / 5802

页数：7

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