A general method for detecting protease activity via gelation and its application to artificial clotting

被引：70

作者：

Bremmer, Steven C. ^{[3
,4
]}

Chen, Jing ^{[1
,2
]}

McNeil, Anne J. ^{[1
,2
]}

Soellner, Matthew B. ^{[3
,4
]}

机构：

[1] Univ Michigan, Dept Chem & Macromol Sci, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Engn Program, Ann Arbor, MI 48109 USA

[3] Univ Michigan, Dept Med Chem, Ann Arbor, MI 48109 USA

[4] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 44期

基金：

美国国家卫生研究院;

关键词：

SUPRAMOLECULAR HYDROGELS; ENZYMATIC HYDROGELATION; FORM NANOFIBERS; PEPTIDE; DERIVATIVES; MOLECULES; SCAFFOLDS; RELEASE; INDUCE; SYSTEM;

D O I：

10.1039/c2cc31537h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A modular system for detecting protease activity via enzyme-triggered gel formation is described. Protease-specific recognition sequences are utilized to achieve enzyme specificity. Artificial blood clotting is demonstrated by activating endogenous thrombin to trigger gelation in fibrinogen-deficient blood plasma.

引用

页码：5482 / 5484

页数：3

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