Basic fibroblast growth factor supports undifferentiated human embryonic stem cell growth without conditioned medium

被引：287

作者：

Xu, CH ^{[1
]}

Rosler, E ^{[1
]}

Jiang, JJ ^{[1
]}

Lebkowski, JS ^{[1
]}

Gold, JD ^{[1
]}

O'Sullivan, C ^{[1
]}

Delavan-Boorsma, K ^{[1
]}

Mok, M ^{[1
]}

Bronstein, A ^{[1
]}

Carpenter, MK ^{[1
]}

机构：

[1] Geron Corp, Menlo Pk, CA 94025 USA

来源：

STEM CELLS | 2005年 / 23卷 / 03期

关键词：

human embryonic stem cells; growth factors; telomerase; stem cell markers; differentiation;

D O I：

10.1634/stemcells.2004-0211

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Previous studies have shown that prolonged propagation of undifferentiated human embryonic stem cells (hESCs) requires conditioned medium from mouse embryonic feeders (MEF-CM) as well as matrix components. Because hESCs express growth factor receptors, including those for basic fibroblast growth factor (bFGF), stem cell factor (SCF), and fetal liver tyrosine kinase-3 ligand (Flt3L), we evaluated these and other growth factors for their ability to maintain undifferentiated hESCs in the absence of conditioned medium. We found cultures maintained in bFGF alone or in combination with other factors showed characteristics similar to MEF-CM control cultures, including morphology, surface marker and transcription factor expression, telomerase activity, differentiation, and karyotypic stability. In contrast, cells in media containing Flt-3L, thrombopoietin, and SCF, individually or in combination, showed almost complete differentiation after 6 weeks in culture. These data demonstrate that hESCs can be maintained in nonconditioned medium using growth factors.

引用

页码：315 / 323

页数：9

共 55 条

[21] Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes [J].