Production of intergeneric hybrids between Brassica and Sinapis species by means of embryo rescue techniques

Reciprocal crosses were made to produce intergeneric hybrids among seven species of Brassica and three species of Sinapis with the aid of embryo rescue techniques. Ovule culture showed better responses in terms of hybrid plant development. Altogether, hybrids from 8 different combinations were obtained from the crosses between B. campestris var. trilocularis x S, turgida, B. campestris var. pekinensis X S. arvensis, S. arvensis x B. campestris var. pekinensis, B. oleracea var. alboglabra x S. alba, B. oleracea var, alboglabra x S. turgida, B. carinata x S. alba, B. carinata x S. arvensis, and B. carinata x S. turgida. Reciprocal crosses yielded no hybrid except in the combination of S. arvensis x B. campestris var. pekinensis. Among them hybrids from 6 combinations were established in the glass house. The hybridity of the plants was confirmed by morphological characters, pollen stainability, chromosome number and by isozyme analysis. Hybrids of four combinations out of six turned out as true hybrids, one as sesquidiploids and the another one as false hybrid plant.