Prepore for a breakthrough

被引:25
作者
Bayley, H [1 ]
Jayasinghe, L [1 ]
Wallace, M [1 ]
机构
[1] Univ Oxford, Dept Chem, Chem Res Lab, Oxford OX1 3TA, England
关键词
D O I
10.1038/nsmb0505-385
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A key to understanding bacterial pathogenicity is the mechanism by which water-soluble protein toxins assemble on cell membranes to form oligomeric bilayer-spanning pores. The recent reconstructions from cryo-electron micrographs of three-dimensional pore and prepore structures of the cholesterol-dependent toxin pneumolysin shed new light on the later steps of the assembly of large toxin pores.
引用
收藏
页码:385 / 386
页数:2
相关论文
共 25 条
[1]   Vertical collapse of a cytolysin prepore moves its transmembrane β-hairpins to the membrane [J].
Czajkowsky, DM ;
Hotze, EM ;
Shao, ZF ;
Tweten, RK .
EMBO JOURNAL, 2004, 23 (16) :3206-3215
[2]   The heptameric prepore of a Staphylococcal alpha-hemolysin mutant in lipid bilayers imaged by atomic force microscopy [J].
Fang, Y ;
Cheley, S ;
Bayley, H ;
Yang, J .
BIOCHEMISTRY, 1997, 36 (31) :9518-9522
[3]   Redefining cholesterol's role in the mechanism of the cholesterol's-dependent cytolysins [J].
Giddings, KS ;
Johnson, AE ;
Tweten, RK .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (20) :11315-11320
[4]   Crystal structure of leucotoxin S component -: New insight into the staphylococcal β-barrel pore-forming toxins [J].
Guillet, V ;
Roblin, P ;
Werner, S ;
Coraiola, M ;
Menestrina, G ;
Monteil, H ;
Prévost, G ;
Mourey, L .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (39) :41028-41037
[5]   Arresting pore formation of a cholesterol-dependent cytolysin by disulfide trapping synchronizes the insertion of the transmembrane ß-sheet from a prepore intermediate [J].
Hotze, EM ;
Wilson-Kubalek, EM ;
Rossjohn, J ;
Parker, MW ;
Johnson, AE ;
Tweten, RK .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (11) :8261-8268
[6]   A bacterial big-MAC attack [J].
Iacovache, I ;
van der Goot, FG .
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2004, 11 (12) :1163-1164
[7]   Arresting and releasing Staphylococcal α-hemolysin at intermediate stages of pore formation by engineered disulfide bonds [J].
Kawate, T ;
Gouaux, E .
PROTEIN SCIENCE, 2003, 12 (05) :997-1006
[8]   The identification and structure of the membrane-spanning domain of the Clostridium septicum alpha toxin [J].
Melton, JA ;
Parker, MW ;
Rossjohn, J ;
Buckley, JT ;
Tweten, RK .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (14) :14315-14322
[9]   Anthrax protective antigen: Prepore-to-pore conversion [J].
Miller, CJ ;
Elliott, JL ;
Collier, RJ .
BIOCHEMISTRY, 1999, 38 (32) :10432-10441
[10]   Single-molecule imaging of cooperative assembly of γ-hemolysin on erythrocyte membranes [J].
Nguyen, VT ;
Kamio, Y ;
Higuchi, H .
EMBO JOURNAL, 2003, 22 (19) :4968-4979