Prepore for a breakthrough

被引：25

作者：

Bayley, H ^{[1
]}

Jayasinghe, L ^{[1
]}

Wallace, M ^{[1
]}

机构：

[1] Univ Oxford, Dept Chem, Chem Res Lab, Oxford OX1 3TA, England

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2005年 / 12卷 / 05期

关键词：

D O I：

10.1038/nsmb0505-385

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A key to understanding bacterial pathogenicity is the mechanism by which water-soluble protein toxins assemble on cell membranes to form oligomeric bilayer-spanning pores. The recent reconstructions from cryo-electron micrographs of three-dimensional pore and prepore structures of the cholesterol-dependent toxin pneumolysin shed new light on the later steps of the assembly of large toxin pores.

引用

页码：385 / 386

页数：2

共 25 条

[11] Controlling pore assembly of staphylococcal γ-haemolysin by low temperature and by disulphide bond formation in double-cysteine LukF mutants [J].

Nguyen, VT ;

Higuchi, H ;

Kamio, Y .

MOLECULAR MICROBIOLOGY, 2002, 45 (06) :1485-1498

[12]

Olson R, 1999, NAT STRUCT BIOL, V6, P134

[13] Vibrio cholerae cytolysin is composed of an α-hemolysin-like core [J].

Olson, R ;

Gouaux, E .

PROTEIN SCIENCE, 2003, 12 (02) :379-383

[14] Assembly mechanism of the oligomeric streptolysin O pore: the early membrane lesion is lined by a free edge of the lipid membrane and is extended gradually during oligomerization [J].

Palmer, M ;

Harris, R ;

Freytag, C ;

Kehoe, M ;

Tranum-Jensen, J ;

Bhakdi, S .

EMBO JOURNAL, 1998, 17 (06) :1598-1605

[15] The structure of a Staphylococcus aureus leucocidin component (LukF-PV) reveals the fold of the water-soluble species of a family of transmembrane pore-forming toxins [J].

Pédelacq, JD ;

Maveyraud, L ;

Prévost, G ;

Baba-Moussa, L ;

González, A ;

Courcelle, E ;

Shepard, W ;

Monteil, H ;

Samama, JP ;

Mourey, L .

STRUCTURE, 1999, 7 (03) :277-287

[16] Membrane-dependent conformational changes initiate cholesterol-dependent cytolysin oligomerization and intersubunit β-strand alignment [J].

Ramachandran, R ;

Tweten, RK ;

Johnson, AE .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2004, 11 (08) :697-705

[17] Structure of a cholesterol-binding, thiol-activated cytolysin and a model of its membrane form [J].

Rossjohn, J ;

Feil, SC ;

McKinstry, WJ ;

Tweten, RK ;

Parker, MW .

CELL, 1997, 89 (05) :685-692

[18] Dominant-negative mutants of a toxin subunit: An approach to therapy of anthrax [J].

Sellman, BR ;

Mourez, M ;

Collier, RJ .

SCIENCE, 2001, 292 (5517) :695-697

[19] Generation of a membrane-bound, oligomerized pre-pore complex is necessary for pore formation by Clostridium septicum alpha toxin [J].

Sellman, BR ;

Kagan, BL ;

Tweten, RK .

MOLECULAR MICROBIOLOGY, 1997, 23 (03) :551-558

[20] Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore [J].

Song, LZ ;

Hobaugh, MR ;

Shustak, C ;

Cheley, S ;

Bayley, H ;

Gouaux, JE .

SCIENCE, 1996, 274 (5294) :1859-1866

← 1 2 3 →