Conditional expression of MCM7 increases tumor growth without altering DNA replication activity

被引：15

作者：

Yoshida, K ^{[1
]}

Inoue, I ^{[1
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Minato Ku, Tokyo 1088639, Japan

来源：

FEBS LETTERS | 2003年 / 553卷 / 1-2期

关键词：

MCM7; DNA replication; homologous recombination; tetracycline-repressible promoter; tumor growth;

D O I：

10.1016/S0014-5793(03)01018-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The minichromosome maintenance (MCM) 2-7 complex is a putative DNA helicase complex that facilitates the initiation of DNA replication. Here, we generated a cell line MCM7(+/-)/MCM7-FLAG, in which one allele of MCM7 is mutated whereas a tetracycline-repressible promoter could manipulate the expression of exogenous MCM7 protein. Overexpressed MCM7 protein supports efficient DNA replication of Epstein-Barr virus oriP and rapid formation of tumors in nude mice without altering the activity of cellular DNA replication. This system provides a unique setting for studying the function of MCM7 and for screening for potential therapeutics for malignant tumors. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：213 / 217

页数：5

共 26 条

[21] Requirement of replication licensing for the dyad symmetry element-dependent replication of the Epstein-Barr virus oriP minichromosome [J].

Shirakata, M ;

Imadome, K ;

Hirai, K .

VIROLOGY, 1999, 263 (01) :42-54

[22] BM28, A HUMAN MEMBER OF THE MCM2-3-5 FAMILY, IS DISPLACED FROM CHROMATIN DURING DNA-REPLICATION [J].

TODOROV, IT ;

ATTARAN, A ;

KEARSEY, SE .

JOURNAL OF CELL BIOLOGY, 1995, 129 (06) :1433-1445

[23] The hexameric eukaryotic MCM helicase: Building symmetry from nonidentical parts [J].

Tye, BK ;

Sawyer, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (45) :34833-34836

[24] MCM proteins in DNA replication [J].

Tye, BK .

ANNUAL REVIEW OF BIOCHEMISTRY, 1999, 68 :649-686

[25]

You ZY, 1999, MOL CELL BIOL, V19, P8003

[26] Mcm2 and Mcm3 are constitutive nuclear proteins that exhibit distinct isoforms and bind chromatin during specific cell cycle stages of Saccharomyces cerevisiae [J].

Young, MR ;

Tye, BK .

MOLECULAR BIOLOGY OF THE CELL, 1997, 8 (08) :1587-1601

← 1 2 3 →