Mathematical analysis of affinity membrane chromatography

A mathematical model including convection, diffusion and Freundlich adsorption is developed. To examine the validity of the model, the affinity membranes were prepared by coating chitosan on the nylon membranes, a ligand of poly-L-lysine was bound to the chitoan-coating membranes, and the adsorption behavior of bilirubin through the stacked affinity membranes was investigated. The agreements between the theoretical and experimental results are exceptional. Using our new model, we show that: (1) As Pe increases, the breakthrough curves become sharper. For Pe greater than 30, the effect of axial diffusion is insignificant; (2) As m increases, the time of total saturation is delayed and the loading capacity at the point of breakthrough is increased; (3) As n decreases, the time of total saturation is delayed and the loading capacity at the point of breakthrough is increased; (4) As r increases, both the time of total saturation and the loading capacity at the point of breakthrough are increased; (5) adsorption rate influences the time of total saturation strongly but contributes little to the loading capacity. (c) 2005 Elsevier B.V. All rights reserved.