Overexpression of recombinant proteins with a C-terminal thiocarboxylate: Implications for protein semisynthesis and thiamin biosynthesis

被引：45

作者：

Kinsland, C ^{[1
]}

Taylor, SV ^{[1
]}

Kelleher, NL ^{[1
]}

McLafferty, FW ^{[1
]}

Begley, TP ^{[1
]}

机构：

[1] Cornell Univ, Baker Lab, Dept Chem & Chem Biol, Ithaca, NY 14853 USA

来源：

PROTEIN SCIENCE | 1998年 / 7卷 / 08期

关键词：

C-terminal thiocarboxylate; protein semisynthesis; recombinant proteins; thiamin biosynthesis;

D O I：

10.1002/pro.5560070821

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A facile and rapid method for the production of protein C-terminal thiocarboxylates on DNA-encoded polypeptides is described. This method, which relies on the mechanism of the cleavage reaction of intein-containing fusion proteins, can produce multi-milligram quantities of protein C-terminal thiocarboxylate quickly and inexpensively. The utility of this method for protein semisynthesis and implications for studies on the biosynthesis of thiamin are discussed.

引用

页码：1839 / 1842

页数：4

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