A simple photo-affinity labeling protocol

被引：13

作者：

Li, HY ^{[1
]}

Liu, Y ^{[1
]}

Fang, K ^{[1
]}

Nakanishi, K ^{[1
]}

机构：

[1] Columbia Univ, Dept Chem, New York, NY 10027 USA

来源：

CHEMICAL COMMUNICATIONS | 1999年 / 04期

关键词：

D O I：

10.1039/a809507h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

After photo-crosslinking and proteolysis of a photoaffinity labeled ligand to its receptor, the ester group incorporated in the ligand is cleaved by an amine with specific functionality, which displaces the bulk of the ligand from the peptide fragments, and facilitates subsequent tandem mass spectrometric sequencing.

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页码：365 / 366

页数：2

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