Demonstration of intrinsic efflux activity of Escherichia coli K-12 AG100 by an automated ethidium bromide method

被引：133

作者：

Viveiros, Miguel ^{[1
]}

Martins, Ana ^{[1
,2
]}

Paixao, Laura ^{[1
]}

Rodrigues, Liliana ^{[1
,2
]}

Martins, Marta ^{[1
,2
]}

Couto, Isabel ^{[1
,3
]}

Faehnrich, Eva ^{[4
]}

Kern, Winfried V. ^{[4
]}

Amaral, Leonard ^{[1
,2
]}

机构：

[1] Univ Nova Lisboa, Inst Higiene & Med Trop, Unit Mycobacteriol, P-1349008 Lisbon, Portugal

[2] Univ Nova Lisboa, Inst Higiene & Med Trop, UPMM, P-1349008 Lisbon, Portugal

[3] Univ Nova Lisboa, Fac Ciencias & Tecnol, CREM, P-2829516 Caparica, Portugal

[4] Univ Hosp, Ctr Infect Dis & Travel Med, D-79106 Freiburg, Germany

来源：

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 2008年 / 31卷 / 05期

关键词：

bacterial efflux pumps; efflux pump inhibitors; ethidium bromide; fluorometry; Escherichia coli; automated method;

D O I：

10.1016/j.ijantimicag.2007.12.015

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Demonstration of efflux of ethidium bromide (EtBr) has been made for over 30 bacterial species, usually by showing enhanced efflux in multidrug-resistant strains that was then abolished by inactivating efflux pumps. Here we present a relatively simple automated method that employs EtBr as an efflux pump substrate for the demonstration of intrinsic efflux activity in Escherichia coli K-12 AG100. The method uses the Rotor-Gene 3000 (TM) instrument for real-time fluorometric measurement of EtBr accumulation under conditions that limit energy (absence of glucose, low temperature) and of EtBr extrusion under optimum conditions. The method can be used for screening compound libraries for efflux inhibiting capacity. (C) 2008 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

引用

页码：458 / 462

页数：5

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