Production of cecropin A in transgenic rice plants has an impact on host gene expression

被引:47
作者
Campo, Sonia [1 ]
Manrique, Silvia [1 ]
Garcia-Martinez, Jose [2 ]
San Segundo, Blanca [1 ]
机构
[1] CSIC, Inst Mol Biol, Dept Mol Genet, Consorcio IRTA Lab Genet Mol Vegetal, ES-08034 Barcelona, Spain
[2] Univ Valencia, SCSIE, Lab Chips DNA, E-46100 Valencia, Spain
关键词
blast; cecropin; endoplasmic reticulum; macroarray; Oryza sativa; oxidative stress; plant defence;
D O I
10.1111/j.1467-7652.2008.00339.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Expression of the cecropin A gene in rice confers resistance to the rice blast fungus Magnaporthe oryzae. In this study, a polymerase chain reaction-based suppression subtractive hybridization approach was used to generate a cDNA macroarray from the elite japonica rice (Oryza sativa L.) cultivar 'Senia'. Gene expression studies revealed that the expression of components of the protein secretory and vesicular transport machinery is co-ordinately activated at the pre-invasive stage of infection of rice by the blast fungus. Comparisons of gene expression between wild-type and cecropin A plants revealed the over-expression of genes involved in protection against oxidative stress in transgenic plants in the absence of the pathogen, which correlated well with the tolerance of these plants to oxidative stress. A subcellular fractionation analysis suggested that cecropin A accumulates in the endoplasmic reticulum in cecropin A rice. Moreover, a large number of genes related to the processes of synthesis, folding and stabilization of proteins that enter into the secretory pathway are over-expressed in cecropin A rice, confirming that these plants constitutively express the unfolded protein response. Transgenic expression of cecropin A in rice has an effect on the transcriptional reprogramming that accompanies plant adaptation to fungal infection. Overall, this study provides evidence for transgene-induced changes in gene expression in cecropin A rice under both optimal growth conditions and stress conditions imposed by fungal infection. The data also indicate that resistance to blast in cecropin A rice may be the consequence of a combination of the antifungal activity of cecropin A and cecropin A-mediated over-expression of rice genes.
引用
收藏
页码:585 / 608
页数:24
相关论文
共 39 条
[1]  
Alberola TM, 2004, INT MICROBIOL, V7, P199
[2]   Stable expression of a defense-related gene in wheat epidermis under transcriptional control of a novel promoter confers pathogen resistance [J].
Altpeter, F ;
Varshney, A ;
Abderhalden, O ;
Douchkov, D ;
Sautter, C ;
Kumlehn, J ;
Dudler, R ;
Schweizer, P .
PLANT MOLECULAR BIOLOGY, 2005, 57 (02) :271-283
[3]   Profilin tyrosine phosphorylation in poly-L-proline-binding regions inhibits binding to phosphoinositide 3-kinase in Phaseolus vulgaris [J].
Aparicio-Fabre, Rosaura ;
Guillen, Gabriel ;
Estrada, Georgina ;
Olivares-Grajales, Juan ;
Gurrola, Georgina ;
Sanchez, Federico .
PLANT JOURNAL, 2006, 47 (04) :491-500
[4]   Transgenesis has less impact on the transcriptome of wheat grain than conventional breeding [J].
Baudo, Maria Marcela ;
Lyons, Rebecca ;
Powers, Stephen ;
Pastori, Gabriela M. ;
Edwards, Keith J. ;
Holdsworth, Michael J. ;
Shewry, Peter R. .
PLANT BIOTECHNOLOGY JOURNAL, 2006, 4 (04) :369-380
[5]   Constitutive expression of ETHYLENE-RESPONSE-FACTOR1 in Arabidopsis confers resistance to several necrotrophic fungi [J].
Berrocal-Lobo, M ;
Molina, A ;
Solano, R .
PLANT JOURNAL, 2002, 29 (01) :23-32
[6]   Lipid rafts in plants [J].
Bhat, RA ;
Panstruga, R .
PLANTA, 2005, 223 (01) :5-19
[7]   Peroxidase-dependent apoplastic oxidative burst in Arabidopsis required for pathogen resistance [J].
Bindschedler, Laurence V. ;
Dewdney, Julia ;
Blee, Kris A. ;
Stone, Julie M. ;
Asai, Tsuneaki ;
Plotnikov, Julia ;
Denoux, Carine ;
Hayes, Tezni ;
Gerrish, Chris ;
Davies, Dewi R. ;
Ausubel, Frederick M. ;
Bolwell, G. Paul .
PLANT JOURNAL, 2006, 47 (06) :851-863
[8]   Analysis of detergent-resistant membranes in Arabidopsis. Evidence for plasma membrane lipid rafts [J].
Borner, GHH ;
Sherrier, DJ ;
Weimar, T ;
Michaelson, LV ;
Hawkins, ND ;
MacAskill, A ;
Napier, JA ;
Beale, MH ;
Lilley, KS ;
Dupree, P .
PLANT PHYSIOLOGY, 2005, 137 (01) :104-116
[9]   TRANSGENIC PLANTS WITH ENHANCED RESISTANCE TO THE FUNGAL PATHOGEN RHIZOCTONIA-SOLANI [J].
BROGLIE, K ;
CHET, I ;
HOLLIDAY, M ;
CRESSMAN, R ;
BIDDLE, P ;
KNOWLTON, S ;
MAUVAIS, CJ ;
BROGLIE, R .
SCIENCE, 1991, 254 (5035) :1194-1197
[10]   The defense response of germinating maize embryos against fungal infection:: A proteomics approach [J].
Campo, S ;
Carrascal, M ;
Coca, M ;
Abián, J ;
San Segundo, B .
PROTEOMICS, 2004, 4 (02) :383-396