Proteomic characterization of Yersinia pestis virulence

被引：57

作者：

Chromy, BA ^{[1
]}

Choi, MW ^{[1
]}

Murphy, GA ^{[1
]}

Gonzales, AD ^{[1
]}

Corzett, CH ^{[1
]}

Chang, BC ^{[1
]}

Fitch, JP ^{[1
]}

McCutchen-Maloney, SL ^{[1
]}

机构：

[1] Lawrence Livermore Natl Lab, Biosci Directorate, Livermore, CA 94550 USA

来源：

JOURNAL OF BACTERIOLOGY | 2005年 / 187卷 / 23期

关键词：

D O I：

10.1128/JB.187.23.8172-8180.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique proteins, were identified by mass spectrometry. Differences in expression were observed for several virulence-associated factors, including catalase-peroxidase (KatY), murine toxin (Ymt), plasminogen activator (Pla), and F1 capsule antigen (Cafl), as well as several putative virulence factors and membrane-bound and metabolic proteins. Differentially expressed proteins not previously reported to contribute to virulence are candidates for more detailed mechanistic studies, representing potential new virulence determinants.

引用

页码：8172 / 8180

页数：9

共 83 条

[1] A novel experimental design for comparative two-dimensional gel analysis: Two-dimensional difference gel electrophoresis incorporating a pooled internal standard [J].

Alban, A ;

David, SO ;

Bjorkesten, L ;

Andersson, C ;

Sloge, E ;

Lewis, S ;

Currie, I .

PROTEOMICS, 2003, 3 (01) :36-44

[2] Immunochemical properties of a 60 kDa cell surface-associated heat shock-protein (Hsp60) from Helicobacter pylori [J].

Amini, HR ;

Ascencio, F ;

CruzVillacorta, A ;

RuizBustos, E ;

Wadstrom, T .

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 1996, 16 (3-4) :163-172

[3]

Amini HR, 1996, FEMS IMMUNOL MED MIC, V16, P247

[4] Intraspecific diversity of Yersinia pestis [J].

Anisimov, AP ;

Lindler, LE ;

Pier, GB .

CLINICAL MICROBIOLOGY REVIEWS, 2004, 17 (02) :434-+

[5] Yersinia enterocolitica ClpB affects levels of invasin and motility [J].

Badger, JL ;

Young, BM ;

Darwin, AJ ;

Miller, VL .

JOURNAL OF BACTERIOLOGY, 2000, 182 (19) :5563-5571

[6] Intracellular signalling and cytoskeletal rearrangement involved in Yersinia pestis plasminogen activator (Pla) mediated HeLa cell invasion [J].

Benedek, O ;

Nagy, G ;

Emody, L .

MICROBIAL PATHOGENESIS, 2004, 37 (01) :47-54

[7]

Bergmann SN, 2004, INDIAN J MED RES, V119, P29

[8] PASTEURELLA PESTIS - ROLE OF PESTICIN I AND IRON IN EXPERIMENTAL PLAGUE [J].

BRUBAKER, RR ;

BEESLEY, ED ;

SURGALLA, MJ .

SCIENCE, 1965, 149 (3682) :422-&

[9]

BRUBAKER RR, 2001, MOL MED MICROBIOLOGY, V3, P2033

[10] Auto, a surface associated autolysin of Listeria monocytogenes required for entry into eukaryotic cells and virulence [J].

Cabanes, D ;

Dussurget, O ;

Dehoux, P ;

Cossart, P .

MOLECULAR MICROBIOLOGY, 2004, 51 (06) :1601-1614

← 1 2 3 4 5 6 7 8 9 →