Proteomic characterization of Yersinia pestis virulence

被引：57

作者：

Chromy, BA ^{[1
]}

Choi, MW ^{[1
]}

Murphy, GA ^{[1
]}

Gonzales, AD ^{[1
]}

Corzett, CH ^{[1
]}

Chang, BC ^{[1
]}

Fitch, JP ^{[1
]}

McCutchen-Maloney, SL ^{[1
]}

机构：

[1] Lawrence Livermore Natl Lab, Biosci Directorate, Livermore, CA 94550 USA

来源：

JOURNAL OF BACTERIOLOGY | 2005年 / 187卷 / 23期

关键词：

D O I：

10.1128/JB.187.23.8172-8180.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique proteins, were identified by mass spectrometry. Differences in expression were observed for several virulence-associated factors, including catalase-peroxidase (KatY), murine toxin (Ymt), plasminogen activator (Pla), and F1 capsule antigen (Cafl), as well as several putative virulence factors and membrane-bound and metabolic proteins. Differentially expressed proteins not previously reported to contribute to virulence are candidates for more detailed mechanistic studies, representing potential new virulence determinants.

引用

页码：8172 / 8180

页数：9

共 83 条

[61] Type III protein secretion in Yersinia species [J].

Ramamurthi, KS ;

Schneewind, O .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2002, 18 :107-133

[62] Expression, characterization, and mutagenesis of the Yersinia pestis murine toxin, a phospholipase D superfamily member [J].

Rudolph, AE ;

Stuckey, JA ;

Zhao, Y ;

Matthews, HR ;

Patton, WA ;

Moss, J ;

Dixon, JE .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (17) :11824-11831

[63] POSTTRANSLATIONAL REGULATION OF LCR PLASMID-MEDIATED PEPTIDES IN PESTICINOGENIC YERSINIA-PESTIS [J].

SAMPLE, AK ;

BRUBAKER, RR .

MICROBIAL PATHOGENESIS, 1987, 3 (04) :239-248

[64] Differential expression of the enolase gene under in vivo versus in vitro growth conditions of Aeromonas hydrophila [J].

Sha, J ;

Galindo, CL ;

Pancholi, V ;

Popov, VL ;

Zhao, Y ;

Houston, CW ;

Chopra, AK .

MICROBIAL PATHOGENESIS, 2003, 34 (04) :195-204

[65] Escherichia coli outer membrane protein A adheres to human brain microvascular endothelial cells [J].

Shin, S ;

Lu, GS ;

Cal, M ;

Kim, KS .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 330 (04) :1199-1204

[66] RECOMBINANT CAPSULAR ANTIGEN (FRACTION-1) FROM YERSINIA-PESTIS INDUCES A PROTECTIVE ANTIBODY-RESPONSE IN BALB/C MICE [J].

SIMPSON, WJ ;

THOMAS, RE ;

SCHWAN, TG .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1990, 43 (04) :389-396

[67] New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase [J].

Sirover, MA .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1999, 1432 (02) :159-184

[68] PLASMINOGEN-ACTIVATOR COAGULASE GENE OF YERSINIA-PESTIS IS RESPONSIBLE FOR DEGRADATION OF PLASMID-ENCODED OUTER-MEMBRANE PROTEINS [J].

SODEINDE, OA ;

SAMPLE, AK ;

BRUBAKER, RR ;

GOGUEN, JD .

INFECTION AND IMMUNITY, 1988, 56 (10) :2749-2752

[69] A SURFACE PROTEASE AND THE INVASIVE CHARACTER OF PLAGUE [J].

SODEINDE, OA ;

SUBRAHMANYAM, YVBK ;

STARK, K ;

QUAN, T ;

BAO, YD ;

GOGUEN, JD .

SCIENCE, 1992, 258 (5084) :1004-1007

[70] GENETIC-ANALYSIS OF THE 9.5-KILOBASE VIRULENCE PLASMID OF YERSINIA-PESTIS [J].

SODEINDE, OA ;

GOGUEN, JD .

INFECTION AND IMMUNITY, 1988, 56 (10) :2743-2748

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