The Polydispersity of αB-Crystallin Is Rationalized by an Interconverting Polyhedral Architecture

被引：85

作者：

Baldwin, Andrew J. ^{[1
,2
,3
,6
]}

Lioe, Hadi ^{[5
]}

Hilton, Gillian R. ^{[5
]}

Baker, Lindsay A. ^{[2
,4
,6
]}

Rubinstein, John L. ^{[2
,4
,6
]}

Kay, Lewis E. ^{[1
,2
,3
,6
]}

Benesch, Justin L. P. ^{[5
]}

机构：

[1] Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A8, Canada

[2] Univ Toronto, Dept Biochem, Toronto, ON M5S 1A8, Canada

[3] Univ Toronto, Dept Chem, Toronto, ON M5S 1A8, Canada

[4] Univ Toronto, Dept Med Biophys, Toronto, ON M5S 1A8, Canada

[5] Univ Oxford, Phys & Theoret Chem Lab, Dept Chem, Oxford OX1 3QZ, England

[6] Hosp Sick Children, Res Inst, Mol Struct & Funct Program, Toronto, ON M5G 1X8, Canada

来源：

STRUCTURE | 2011年 / 19卷 / 12期

基金：

加拿大自然科学与工程研究理事会;

关键词：

HEAT-SHOCK PROTEINS; MASS-SPECTROMETRY REVEALS; QUATERNARY DYNAMICS; GAS-PHASE; MACROMOLECULAR ASSEMBLIES; STRUCTURAL BIOLOGY; SUBUNIT EXCHANGE; A-CRYSTALLIN; STATE NMR; COMPLEXES;

D O I：

10.1016/j.str.2011.09.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report structural models for the most abundant oligomers populated by the polydisperse molecular chaperone alpha B-crystallin. Subunit connectivity is determined by using restraints obtained from nuclear magnetic resonance spectroscopy and mass spectrometry measurements, enabling the construction of various oligomeric models. These candidate structures are filtered according to their correspondence with ion-mobility spectrometry data and cross-validated by using electron microscopy. The ensuing best-fit structures reveal the polyhedral architecture of alpha B-crystallin oligomers, and provide a rationale for their polydispersity and facile interconversion.

引用

页码：1855 / 1863

页数：9

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