Detection of chlorinated DNA and RNA nucleosides by HPLC coupled to tandem mass spectrometry as potential biomarkers of inflammation

被引：61

作者：

Badouard, C

Masuda, M

Nishino, H

Cadet, J

Favier, A

Ravanat, JL ^{[1
]}

机构：

[1] CEA, SCIB, DRFMC, Lab Les Acides Nucl, 17 Rue Martyrs, F-38054 Grenoble, France

[2] Kyoto Prefectural Univ Med, Dept Biochem & Mol Biol, Kamigyo Ku, Kyoto 6028566, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 827卷 / 01期

关键词：

oxidative stress; DNA damage; RNA damage; hypochlorous acid; tandem mass spectrometry; human biomarkers;

D O I：

10.1016/j.jchromb.2005.03.025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Upon inflammation, activated neutrophils secrete myeloperoxidase, an enzyme able to generate hypochlorous acid (HOCI) from hydrogen peroxide and chloride ions. An analytical method, involving HPLC coupled to electrospray tandem mass spectrometry, has been set-up to detect low levels of HOCI-induced nucleic acids lesions, including both ribo and 2'-deoxyribonucleoside derivatives of 8-chloroguanine, 8-chloroadenine and 5-chlorocytosine. Validation of the developed method was achieved using isolated cells treated with HOCI The method was found to be sensitive enough to allow the measurement of background levels of 5-chloro-2'-deoxycytidine in the DNA of human white blood cells isolated from 7 mL of blood. (c) 2005 Elsevier B.V. All rights reserved.

引用

收藏

页码：26 / 31

页数：6

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