Overexpressing MhNPR1 in transgenic Fuji apples enhances resistance to apple powdery mildew

被引：48

作者：

Chen, Xiu-Kong ^{[1
]}

Zhang, Ji-Yu ^{[2
,3
]}

Zhang, Zhen ^{[1
]}

Du, Xiao-Li ^{[1
]}

Du, Bei-Bei ^{[1
]}

Qu, Shen-Chun ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China

[2] Inst Bot, Nanjing 210014, Jiangsu, Peoples R China

[3] Chinese Acad Sci, Nanjing 210014, Jiangsu, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2012年 / 39卷 / 08期

关键词：

MhNPR1; Powdery mildew pathogen; Systemic acquired resistance; Pathogenesis-related protein; DISEASE-RESISTANCE; CONSTITUTIVE ACTIVATION; DEFENSE RESPONSES; ARABIDOPSIS; GENES; HYPERSENSITIVITY; EXPRESSION; BACTERIAL; SPECTRUM; FUNGAL;

D O I：

10.1007/s11033-012-1655-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fuji is susceptible to fungal diseases like apple powdery mildew. Non-expressor of pathogenesis-related gene 1 (NPR1) plays a key role in regulating salicylic acid (SA)-mediated systemic acquired resistance (SAR). Previous studies show that overexpressing the Malus hupehensis-derived NPR1 (MhNPR1) gene in tobacco induces the transcript expression of pathogenesis-related genes (PRs) and resistance to the fungus Botrytis cinerea. In this study we introduced the MhNPR1 gene into the 'Fuji' apple via Agrobacterium-mediated transformation. Four transgenic apple lines were verified by PCR and RT-PCR. The semi-quantitative RT-PCR results showed that transcript overexpression of the MhNPR1 gene induced the expression of MdPRs and MdMLO genes known to interact with powdery mildew. Furthermore, the transgenic apple plants resisted infection by apple powdery mildew better than the wild-type plants. As a result, transcript overexpression of the MhNPR1 gene induced SAR and enhanced the Fuji apple's resistance to fungal disease.

引用

页码：8083 / 8089

页数：7

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