On-chip synthesis and label-free assays of oligosaccharide arrays

被引：87

作者：

Ban, Lan ^{[1
,2
]}

Mrksich, Milan ^{[1
,2
]}

机构：

[1] Univ Chicago, Howard Hughes Med Inst, Chicago, IL 60637 USA

[2] Univ Chicago, Dept Chem, Chicago, IL 60637 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 18期

关键词：

biochips; enzymes; glycosides; mass spectrometry;

D O I：

10.1002/anie.200704998

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Sugar coated: A new strategy for solid-support oligosaccharide array synthesis and direct SAMDI-TOF (self-assembled monolayers for matrix-assisted laser desorption-ionization time-of-flight) mass spectrometry detection of the synthesis process is reported (see scheme). It incorporates an on-chip ligand array synthesis, a label-free detection method, and direct application to an enzymatic assay. (Chemical Equation). © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：3396 / 3399

页数：4

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