Peptide-grafted nanodiamonds: Preparation, cytotoxicity and uptake in cells

被引：70

作者：

Vial, Stephanie ^{[1
]}

Mansuy, Christelle ^{[1
]}

Sagan, Sandrine ^{[1
]}

Irinopoulou, Theano ^{[2
]}

Burlina, Fabienne ^{[1
]}

Boudou, Jean-Paul ^{[3
]}

Chassaing, Gerard ^{[1
]}

Lavielle, Solange ^{[1
]}

机构：

[1] Univ Paris 06, CNRS, UMR Synthese 7613, F-75005 Paris, France

[2] Univ Paris 06, INSERM, UMR S 839, Inst Fer Moulin, F-75005 Paris, France

[3] Univ Paris 06, CNRS, UMR Biogeochem Milleux Continentaux 7618, F-75005 Paris, France

来源：

CHEMBIOCHEM | 2008年 / 9卷 / 13期

关键词：

cellular uptake; cytotoxicity; nanostructures; peptides; surface coating;

D O I：

10.1002/cbic.200800247

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nonodiamonds that were prepared by high pressure/high temperature were functionalised with biomolecules for biological applications. Nanodiamonds (NDs, <= 35 nm) that were coated by silanisation or with polyelectrolyte layers were grafted with a fluorescent thiolated peptide via a maleimido function; this led to an aqueous colloidal suspension that was stable for months. These substituted NDs were not cytotoxic for CHO cells. Their capacity to enter mammalian cells, and their localisation inside were ascertained after labelling the nucleus and actin, by examining the cells by confocal, reflected light and fluorescence microscopy.

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页码：2113 / 2119

页数：7

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