A nitrilase from a metagenomic library acts regioselectively on aliphatic dinitriles

被引：42

作者：

Bayer, Sally ^{[1
]}

Birkemeyer, Claudia ^{[2
]}

Ballschmiter, Meike ^{[1
]}

机构：

[1] Univ Leipzig, Inst Biochem, Fac Biosci Pharm & Psychol, Jr Res Grp White Biotechnol, D-04103 Leipzig, Germany

[2] Univ Leipzig, Inst Analyt Chem, Fac Chem & Mineral, D-04317 Leipzig, Germany

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2011年 / 89卷 / 01期

关键词：

Aliphatic nitrilase; Dinitrile; Metagenome; Regioselectivity; ENANTIOSELECTIVE HYDROLYSIS; SELECTIVE HYDROLYSIS; CARBOXYLIC-ACIDS; GENE CLONING; PURIFICATION; EXPRESSION; SOIL; ENZYMES;

D O I：

10.1007/s00253-010-2831-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Several novel nitrilases were selected from meta-genomic libraries using cinnamonitrile and a mixture of six different nitriles as substrates. The nitrilase gene nit1 was expressed in Escherichia coli and the resulting protein was further examined concerning its biochemical properties. Nit1 turned out to be an aliphatic nitrilase favoring dinitriles over mononitriles. Stereochemical analysis revealed that Nit1 converted the dinitrile 2-methylglutaronitrile regioselectively. Hydrolysis at the omega-nitrile group of a dinitrile, such as catalyzed by Nit1, leads to omega-cyanocarboxylic acids, which are important precursors for chemical and pharmaceutical products. Nit1 metabolized 2-methylglutaronitrile to the corresponding omega-cyanocarboxylic acid 4-cyanopentanoic acid can be used for the production of the fine chemical 1,5-dimethyl-2-piperidone.

引用

页码：91 / 98

页数：8

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