HPLC-DAD determination of Metformin in human plasma using derivatization with p-nitrobenzoyl chloride in a biphasic system

被引：69

作者：

Tache, F

David, V

Farca, A

Medvedovici, A

机构：

[1] Univ Bucharest, Fac Chem, Dept Analyt Chem, Bucharest, Romania

[2] Labormed Pharma SA, Analyt Control Lab, Bucharest 6, Romania

来源：

MICROCHEMICAL JOURNAL | 2001年 / 68卷 / 01期

关键词：

metformin; derivatization with p-nitrobenzoyl chloride; human plasma; HPLC-DAD;

D O I：

10.1016/S0026-265X(00)00170-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An optimized method for determination of Metformin at ppb level in human plasma is described. Derivatization of Metformin with p-nitrobenzoyl chloride was carried out in a biphasic system. The derivative extracted in the organic layer (dichloromethane) was concentrated and analyzed by HPLC-DAD, using an isocratic elution (water/methanol mixture 65/35), monitored at 280 nm. Good selectivity against co-extracted matrix components was observed. A detection limit of 10 ppb Metformin in human plasma was reached. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：13 / 19

页数：7

共 8 条

[1] DETERMINATION OF METFORMIN AND PHENFORMIN IN HUMAN-PLASMA AND URINE BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].