Multitasking with molecular dynamics Typhoon: quantifying nucleic acids and autoradiographs

被引：11

作者：

Goodrich, RJ

Ostermeier, GC

Krawetz, SA ^{[1
]}

机构：

[1] Wayne State Univ, Dept Obstet & Gynecol, Detroit, MI 48201 USA

[2] Wayne State Univ, Ctr Mol Med & Genet, Detroit, MI 48201 USA

[3] Wayne State Univ, Ctr Comp Sci, Detroit, MI 48201 USA

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 13期

关键词：

DNA; microplates; PicoGreen; RiboGreen; RNA;

D O I：

10.1023/A:1024154817579

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

With increased sensitivity and specificity, fluorescent assays are rapidly becoming the method of choice for nucleic acid quantification. The utility of the Typhoon scanner has now been extended to accurately measure low levels of DNA and RNA (5 ng ml(-1)) with PicoGreen and RiboGreen dyes. In addition, with a few simple modifications, autoradiographic film images can be scanned and quantified with the Typhoon series of scanners.

引用

页码：1061 / 1065

页数：5

共 6 条

[1] RNA quantitation by fluorescence-based solution assay: RiboGreen reagent characterization [J].