Direct on-column derivatisation in gas chromatography .2. Comparison of various on-column methylation reagents and the development of a new selective methylation reagent

被引：15

作者：

Amijee, M ^{[1
]}

Cheung, J ^{[1
]}

Wells, RJ ^{[1
]}

机构：

[1] AUSTRALIAN GOVT ANALYT LABS,PYMBLE,NSW 2073,AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 738卷 / 01期

关键词：

derivatization; GC; methylation; benzimidazoles; phenyltrimethylammonium salts; trimethylsulfonium salts; sulfonamides; phenols; fatty acids; chlorophenols; nitrophenols;

D O I：

10.1016/0021-9673(96)00087-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been demonstrated that judicious choice of the derivatisation reagent used for direct on-column methylations can have a profound effect of the products produced, often with little effect on the overall methylation efficiency of the process. Trimethylsulfonium acetate, trimethylsulfonium cyanide and phenyltrimethylammonium acetate are three new selective methylation reagents for direct on-column derivatisation in gas chromatography. Phenyltrimethylammonium acetate is a particularly stable, selective and efficient new direct on-column methylation reagent. This work demonstrates that, for routine on-column methylation where no selectivity is required, phenyltrimethylammonium fluoride (PTMA-F) is as efficient as phenyltrimethylammonium hydroxide. PTMA-F is the preferred reagent because it is neutral and produces less column deterioration than the hydroxide. Where methylation selectivity is required, both phenyltrimethylammonium acetate has very similar efficiency and selectivity as the previously reported cyanide salt. However, the acetate is the more stable and readily utilised than the cyanide and is the best of a number of selective on-column methylation reagents surveyed in this work.

引用

页码：43 / 55

页数：13

共 9 条

[1] Direct on-column derivatisation in gas chromatography .3. On-column benzylation reagents and the development of 3,5-bis(trifluoromethyl)benzyl-dimethylphenylammonium fluoride, an efficient new on-column derivatisation reagent [J].