MreB actin-mediated segregation of a specific region of a bacterial chromosome

被引：319

作者：

Gital, Z ^{[1
]}

Dye, NA

Reisenauer, A

Wachi, M

Shapiro, L

机构：

[1] Stanford Univ, Beckman Ctr, Sch Med, Dept Dev Biol, Stanford, CA 94305 USA

[2] Stanford Univ, Beckman Ctr, Sch Med, Dept Biochem, Stanford, CA 94305 USA

[3] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA

[4] Tokyo Inst Technol, Dept Bioengn, Midori Ku, Yokohama, Kanagawa 2268501, Japan

来源：

CELL | 2005年 / 120卷 / 03期

关键词：

D O I：

10.1016/j.cell.2005.01.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Faithful chromosome segregation is an essential component of cell division in all organisms. The eukaryotic mitotic machinery uses the cytoskeleton to move specific chromosomal regions. To investigate the potential role of the actin-like MreB protein in bacterial chromosome segregation, we first demonstrate that MreB is the direct target of the small molecule A22. We then demonstrate that A22 completely blocks the movement of newly replicated loci near the origin of replication but has no qualitative or quantitative effect on the segregation of other loci if added after origin segregation. MreB selectively interacts, directly or indirectly, with origin-proximal regions of the chromosome, arguing that the origin-proximal region segregates via an MreB-dependent mechanism not used by the rest of the chromosome.

引用

页码：329 / 341

页数：13

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