Chiral discrimination using an immunosensor

被引：100

作者：

Hofstetter, O

Hofstetter, H

Wilchek, M

Schurig, V

Green, BS ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel

[2] Univ Tubingen, Inst Organ Chem, D-72076 Tubingen, Germany

[3] Hebrew Univ Jerusalem, Dept Pharmaceut Chem, IL-91120 Jerusalem, Israel

来源：

NATURE BIOTECHNOLOGY | 1999年 / 17卷 / 04期

关键词：

applied immunology; chiral sensor; amino acid;

D O I：

10.1038/7927

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Based on the stereoselectivity of immunoglobulins, we have developed a new chiral sensor for the detection of low-molecular-weight analytes. Using surface plasmon resonance detection, enantiomers of free, underivatized alpha-amino acids can be monitored in a competitive assay by their interaction with antibodies specific for the chiral center of this class of substances. The sensitivity to the minor enantiomer in nonracemic mixtures exceeds currently available methods; therefore, such immunosensors can readily detect traces of enantiomeric impurities and are attractive for a range of applications in science and industry.

引用

页码：371 / 374

页数：4

共 28 条

[21] Selective complexation and sensitive analysis of charge diffuse cationic species using lipophilic cyclodextrins [J].