Sprouty gene expression is regulated by nerve and FGF6 during regeneration of mouse muscles

被引：10

作者：

Laziz, Iman ^{[1
]}

Armand, Anne-Sophie ^{[1
]}

Pariset, Claude ^{[1
]}

Lecolle, Sylvie ^{[1
]}

Della Gaspera, Bruno ^{[1
]}

Charbonnier, Frederic ^{[1
]}

Chanoine, Christophe ^{[1
]}

机构：

[1] Univ Paris 05, Ctr Univ Saints Peres, Lab Biol Dev & Diff Neuromuscl, UMR 7060,CNRS, F-75720 Paris, France

来源：

GROWTH FACTORS | 2007年 / 25卷 / 03期

关键词：

sprouty; FGF; RTK; myogenesis; regeneration; denervation;

D O I：

10.1080/08977190701723166

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Sprouty (Spry) proteins were identified as negative regulators of fibroblast growth factor (FGF) signaling in vertebrates and invertebrates. Given the importance of the FGFs in myogenesis, we performed cardiotoxin injury- induced regeneration experiments on soleus muscles of both, adult control and FGF6 (-/-) mutant mice and analyzed the accumulation of Spry (1, 2 and 4) transcripts using semi- quantitative and real- time RT- PCR assays and in situ hybridization. We also analyzed the effects of muscle denervation on the accumulation of Spry transcripts. The three Spry genes begin to be expressed as early as the first stages of muscle regeneration and are characterized by distinct expression patterns. Moreover, Spry gene expression was highly and differentially up- regulated, precociously by the lack of FGF6, and belatedly by muscle denervation strongly suggesting that the transient rise of Spry mRNA accumulation was associated to muscle differentiation. Rescue experiments supported the idea of a specific relationship between FGF6 and Spry 2, both being known for their particular involvement in myogenesis.

引用

页码：151 / 159

页数：9

共 29 条

[21] Identification of a dominant negative mutant of sprouty that potentiates fibroblast growth factor-but not epidermal growth factor-induced ERK activation [J].