Simultaneous catalyzing of uricase- and catalase-like reactions of resins modified with metalloporphyrins and its application for determination of uric acid

被引：3

作者：

Odo, J ^{[1
]}

Hashimoto, H

机构：

[1] Okayama Univ Sci, Fac Sci, Okayama 7000005, Japan

[2] Okayama Univ, Fac Pharmaceut Sci, Okayama 7000082, Japan

来源：

ANALYTICAL SCIENCES | 1998年 / 14卷 / 05期

关键词：

uric acid; modified resin; catalytic activity; manganese-tetrakis(4-sulfophenyl)porphin uricase; catalase;

D O I：

10.2116/analsci.14.935

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Some anion-exchange resins modified with water-soluble metalloporphyrins exhibited an ability to catalyze simultaneously both uricase- and catalase-like reactions. The resin modified with manganese-tetrakis(4- sulfophenyl)porphine (Mn-TPPSr), which showed the highest activity among the resins tested, was useful for the indirect determination of uric acid by measuring the amount of formaldehyde produced. The calibration curve was linear over the range from 10 mu g to 200 mu g of uric acid with a relative standard deviation of 1.8% (n=10) for 200 mu g uric acid. Mn-TPPSr was also applied for the determination of uric acid in sera.

引用

页码：935 / 939

页数：5

共 7 条

[1] NEW SENSITIVE AND SPECIFIC TEST FOR DETECTION OF ALDEHYDES - FORMATION OF 6-MERCAPTO-3-SUBSTITUTED-S-TRIAZOLO4,3-B!-S-TETRAZINES [J].