Engineered RNA-mediated resistance to tomato spotted wilt virus is sequence specific

被引：57

作者：

Prins, M

Resende, RD

Anker, C

vanSchepen, A

deHaan, P

Goldbach, R

机构：

[1] AGR UNIV WAGENINGEN,DEPT VIROL,6709 PD WAGENINGEN,NETHERLANDS

[2] S&G SEEDS,DIV TECHNOL,1600 AA ENKHUIZEN,NETHERLANDS

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1996年 / 9卷 / 05期

关键词：

movement; protein; Nicotiana tabacum; nucleoprotein; tospovirus; virus resistance;

D O I：

10.1094/MPMI-9-0416

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transgenic plants were produced that expressed a wide range of randomly chosen sequences of the tripartite tomato spotted wilt virus (TSWV) RNA genome or its complement, Testing the progenies of these plants revealed that only transgenic expression of N or NSM gene sequences resulted in resistance to TSWV.

引用

收藏

页码：416 / 418

页数：3

相关论文

共 22 条

[1] DELAY OF DISEASE DEVELOPMENT IN TRANSGENIC PLANTS THAT EXPRESS THE TOBACCO MOSAIC-VIRUS COAT PROTEIN GENE [J].

ABEL, PP ;

NELSON, RS ;

DE, B ;

HOFFMANN, N ;

ROGERS, SG ;

FRALEY, RT ;

BEACHY, RN .

SCIENCE, 1986, 232 (4751) :738-743

[2] REPLICASE-MEDIATED RESISTANCE TO POTATO-VIRUS-Y IN TRANSGENIC TOBACCO PLANTS [J].

AUDY, P ;

PALUKAITIS, P ;

SLACK, SA ;

ZAITLIN, M .

MOLECULAR PLANT-MICROBE INTERACTIONS, 1994, 7 (01) :15-22

[3] TRANSGENIC RESISTANCE TO PLANT-VIRUSES - INTRODUCTION [J].

BEACHY, RN .

SEMINARS IN VIROLOGY, 1993, 4 (06) :327-328

[4] BINARY AGROBACTERIUM VECTORS FOR PLANT TRANSFORMATION [J].

BEVAN, M .

NUCLEIC ACIDS RESEARCH, 1984, 12 (22) :8711-8721

[5] CHARACTERIZATION OF RNA-MEDIATED RESISTANCE TO TOMATO SPOTTED WILT VIRUS IN TRANSGENIC TOBACCO PLANTS [J].

DEHAAN, P ;

GIELEN, JJL ;

PRINS, M ;

WIJKAMP, IG ;

VANSCHEPEN, A ;

PETERS, D ;

VANGRINSVEN, MQJM ;

GOLDBACH, R .

BIO-TECHNOLOGY, 1992, 10 (10) :1133-1137

[6]

DOUGHERTY WG, 1994, MOL PLANT MICROBE IN, V7, P544, DOI 10.1094/MPMI-7-0554

[7]

English JJ, 1996, PLANT CELL, V8, P179, DOI 10.1105/tpc.8.2.179

[8] ENGINEERED RESISTANCE TO TOMATO SPOTTED WILT VIRUS, A NEGATIVE-STRAND RNA VIRUS [J].

GIELEN, JJL ;

DEHAAN, P ;

KOOL, AJ ;

PETERS, D ;

VANGRINSVEN, MQJM ;

GOLDBACH, RW .

BIO-TECHNOLOGY, 1991, 9 (12) :1363-1367

[9]

Goodwin J, 1996, PLANT CELL, V8, P95, DOI 10.1105/tpc.8.1.95

[10] EXPRESSION AND SUBCELLULAR LOCATION OF THE NSM PROTEIN OF TOMATO SPOTTED WILT VIRUS (TSWV), A PUTATIVE VIRAL MOVEMENT PROTEIN [J].

KORMELINK, R ;

STORMS, M ;

VANLENT, J ;

PETERS, D ;

GOLDBACH, R .

VIROLOGY, 1994, 200 (01) :56-65