Development and characterization of novel tetra-, tri-, and dinucleotide microsatellite markers in rainbow trout (Oncorhynchus mykiss)

被引：72

作者：

Spies, IB

Brasier, DJ

O'Reilly, TL

Seamons, TR

Bentzen, P

机构：

[1] NOAA Fisheries, Alaska Fisheries Sci Ctr, Seattle, WA 98115 USA

[2] Univ Calif San Diego, La Jolla, CA 92093 USA

[3] Bedford Inst Oceanog, Dartmouth, NS B2Y 4A2, Canada

[4] Univ Washington, Marine Mol Biotechnol Lab, Seattle, WA 98105 USA

[5] Dalhousie Univ, Dept Biol, Halifax, NS B3H 4J1, Canada

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 02期

关键词：

enrichment; genome mapping; microsatellites; Oncorhynchus mykiss; population genetics; rainbow trout;

D O I：

10.1111/j.1471-8286.2005.00900.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We discuss the development and characterization of 40 polymorphic rainbow trout (Oncorhynchus mykiss) microsatellite loci. We used enriched libraries to isolate 14 dinucleotide, seven trinucleodide, eight compound di/tetranucleotide, and 11 tetranucleotide loci. These markers will be useful for selective breeding via marker-assisted selection, population genetics studies, parentage analysis, and have already been used for genome mapping.

引用

页码：278 / 281

页数：4

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