Promotion of the growth of Crocus sativus cells and the production of crocin by rare earth elements

被引：63

作者：

Chen, SA ^{[1
]}

Zhao, B ^{[1
]}

Wang, XD ^{[1
]}

Yuan, XF ^{[1
]}

Wang, YC ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 01期

基金：

国家高技术研究发展计划(863计划);

关键词：

biosynthesis; cell growth; crocin; Crocus sativus; rare earth elements;

D O I：

10.1023/B:BILE.0000009455.31817.a5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

La3+ and Ce3+, either singly or a mixture, promoted crocin production of Crocus sativus callus but Nd3+ had little effect and all metal ions were toxic above 100 muM. La3+ (60 muM) promoted growth of callus significantly but increased crocin only slightly. Ce3+ (40 muM) significantly promoted crocin production but had little effect on cell growth. La3+ (60 muM) and Ce3+ (20 muM) together gave the highest dry weight biomass (20.4 g l(-1)), crocin content (4.4 mg g(-1)) and crocin production (90 mg l(-1)) which were, respectively, 1.7-fold, 4.2-fold and 7.1-fold of those without additions. Nd3+ (40 muM) only slightly promoted cell growth and crocin production.

引用

页码：27 / 30

页数：4

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